RNA Interference and Model Organisms
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• Divided into two parts, the first is devoted to exploration of the histroy of RNAi mechanisms and experiment design in model organisms, while the second half contains a lab manual with detailed protocols for performing silencing experiments.
• Includes discussion of microRNAs and therapeutics.
  
   

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Part  1  RNA Interference (RNAi) and Model Organisms
  Chapter  1  Introduction to RNA Silencing
  Chapter  2  Post Transcriptional Gene Silencing in Plants
  Chapter  3  RNAi in Caenorhabditis elegans
  Chapter  4  Genetic Manipulations and RNAi in Drosophila
  Chapter  5  RNAi and Mammalian Systems
  Chapter  6  MicroRNAs
Part  2  Laboratory Sessions on RNA Interference
  Lab  Session 1  Plant Transplantation and DNA Preparation
  Lab  Session 2  Virus-Induced Gene Silencing of Magnesium Chelatase, a Chlorophyll
  Lab  Session 3  Phenotypic Assessment of Gene Silencing in Tobacco Plants, and Initiation of C. elegans Silencing Experiment to Knockdown Transgenic Green Fluorescent Protein Expression via Feeding
  Lab  Session 4  Assessment of Gene Silencing in Plants and in C. elegans
  Lab  Session 5  Evaluation of Gene Silencing in Tobacco Plants and Initiation of Mammalian Cell Culture Silencing Experiment Using Transient Transfection to Deliver Short Hairpin RNA Expression Plasmids to Knockdown an egfp Transgene
  Lab  Session 6  Quantification of Fluorescence of Silenced Mammalian Cells and Initiation of Immunoblot to Assess EGFP Protein Levels
  Lab  Session 7  Analysis of Effect of Silencing on EGFP Protein Levels in Mammalian Cell Culture Cells
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Ideal for the upper-level undergraduate and graduate student, or for use as a professional reference, RNA Interference and Model Organisms helps readers understand the history of RNAi and how it can be applied to model organisms.  Back to top