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DNA Sequencing II: Optimizing Preparation and Cleanup

Author(s): Jan Kieleczawa, PhD, MBA, Wyeth Research - Genomics Department
Details:
  • ISBN-13: 9780763733834
  • ISBN-10:0763733830
  • Hardcover    362 pages      © 2006
Price: International Sales $119.95 US List
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Overview

Kieleczawa's second volume, DNA Sequencing II:  Optimizing the Preparation and Clean-Up, is devoted to the various methods used for extraction, clean-up, quantification, and analysis of DNA.  This volume is divided into four comprehensive sections - DNA Purification, Clean-up of DNA Fragments, Storage of DNA, and Quantifying DNA and RNA - and offers the reader an in-depth presentation of DNA technologies.  The text also touches upon the many tools and software programs that are found in a typical modern biology laboratory.  This fascinating text is a wonderful addition to your molecular biology library.

ShowTable of Contents

Foreword by Richard Gibbs

1.   Preparation of Difficult DNA Templates using Seven Different Commercial Methods

2.   Isolation and Use of Bacterial and P1 Bacteriophage-Derived Artificial Chromosomes

3.   Preparation of Genomic DNA from Animal Tissue

4.   Extraction of Genomic DNA from Plant Tissue

5.   Preparation of cDNA Probes for DNA Microarrays

6.   Isolation of Nucleic acids from Paraffin-Embedded Archival Tissues and other "Difficult" Sources

7.  Comparison of Sequencing Data for DNAs Prepared Using TempliPhi Technology and Traditional Preparation Protocols

8.  Whole Genome Amplification using the Multiple Displacement Amplification Reaction

9.  Solid Phase Nucleic Acid Purification using Magnetic Beads

10. The Sanger Institute High Through-put Sequencing Pipeline

11. Comparison of PCR Product Clean-up Methods for High Through-put Capillary Electrophoresis

12. Evaluation of Methods for Clean-up of DNA Sequencing Reactions

13. Thermostable DNA Polymerases for a Wide Spectrum of Applications: Comparison of a Robust Hybrid TopoTaq to other enzymes

14. Prolonged Storage of Plasmid DNAs Under Different Conditions: Effect on Plasmid Integrity Spectral Characteristics and DNA Sequence Quality

15. The DNA Book - A New Method of the Storage and Distribution of Genome Resources

16. Assessing the Quantity, Purity and Integrity of RNA and DNA Following Nucleic Acid Purification

17.  Essential Software and Other Tools in Modern Biology Laboratories

 

 


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ShowAbout the Author(s)

Jan Kieleczawa, PhD, MBA-Wyeth Research - Genomics Department

Dr. Jan Kieleczawa earned his masters and Ph.D. degrees from the University of Wroclaw (Poland) and his M.B.A. from Oakdale College. Currently, he heads the DNA Sequencing Group at Wyeth Research in Cambridge, MA. His research interest involve developing methods of sequencing numerous types of difficult DNA templates.

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